APPLICATION
Application Note
Recommended Starting Dilutions:
at 1μg of antibody per 200μg of whole cell lysate for IP, & 1:100 for IF
Optimal dilutions should be determined experimentally by the researcher.
Calculated MW
Product Note
Specific for human HEF1 localized in mitotic cells in the spindle and the centrosome. It does not recognize p130Cas. Have not tested it on Sin1
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Immunogen
GST-HEF-1 (82-398aa.)
Purification
Protein G
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
neural precursor cell expressed, developmentally down-regulated 9 , CAS-L , CAS2 , CASL , CASS2 , HEF1
Cellular Localization
Nucleus , Golgi apparatus , Cytoplasm
Background
The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Database
Research Area
REFERENCE
There are currently no references for HEF1 antibody [14A11] (GTX18059). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HEF1 antibody [14A11] (GTX18059). Be the first to share your experience with this product.