Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HEY1 antibody [HL2367] detects HEY1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
HEY1 stained by HEY1 antibody [HL2367] (GTX638573) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.