Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [N3C2], Internal (GTX112584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [N3C2], Internal (GTX112584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [N3C2], Internal (GTX112584) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HHIP antibody [N3C2], Internal detects HHIP protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
HHIP antibody [N3C2], Internal (GTX112584) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

HHIP antibody [N3C2], Internal antibody detects HHIP protein by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde for 15 min.
Green: HHIP protein stained by HHIP antibody [N3C2], Internal antibody (GTX112584) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

HHIP antibody [N3C2], Internal detects HHIP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
HHIP stained by HHIP antibody [N3C2], Internal (GTX112584) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min