ICC/IF analysis of A2058 Cells using GTX22771 HIF1 beta antibody [2B10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

WB analysis of whole cell extracts (30 μg lysate) of HeLa (Lane 1), MCF-7 (Lane 2), NIH/3T3 (lane 3) and PC 12 (Lane 4) using GTX22771 HIF1 beta antibody [2B10].
Dilution : 1:500-3000

ICC/IF analysis of U251 cells using GTX22771 HIF1 beta antibody [2B10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

ICC/IF analysis of NRK cells using GTX22771 HIF1 beta antibody [2B10].
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes
Dilution : 40 μg/ml in staining buffer for 1 hour at room temperature

ICC/IF analysis of HeLa cells using GTX22771 HIF1 beta antibody [2B10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC