Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 beta antibody [GT1141] (GTX00799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

WB analysis of HeLa whole cell lysate using GTX00799 HIF1 beta antibody [GT1141].
Dilution : 1:1000
Loading : 25 μg

IHC-P analysis of hmuan colon tissue section using GTX00799 HIF1 beta antibody [GT1141].
Dlution : 1:100