Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIP1 antibody (GTX131449) diluted at 1:1000.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIP1 antibody (GTX131449) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.