Wild-type (WT) and HPRT knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX101148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX101148) diluted at 1:1000.

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX101148) diluted at 1:500.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX101148) diluted at 1:500.

Immunofluorescence analysis of methanol-fixed A549, using HPRT(GTX101148) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using HPRT(GTX101148) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Zebrafish tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX101148) diluted at 1:500.