HRH1 antibody [HL2783]

Cat. No. GTX639651

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2783
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all HRH1 products

Datasheet

View product citations for antibody GTX639651 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Product Note

This antibody was raised against human HRH1 Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain human of HRH1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histamine receptor H1 , H1-R , H1R , HH1R , hisH1

Cellular Localization

Cell membrane

Background

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. It has been associated with multiple processes, including memory and learning, circadian rhythm, and thermoregulation. It is also known to contribute to the pathophysiology of allergic diseases such as atopic dermatitis, asthma, anaphylaxis and allergic rhinitis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2015]

Database

Gene ID: 3269 HRH1
UniProt: P35367 HRH1

Research Area

DATA IMAGES

GTX639651 IHC-P Image

HRH1 antibody [HL2783] detects HRH1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HRH1 stained by HRH1 antibody [HL2783] (GTX639651) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX639651 WB Image

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL2783] (GTX639651) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations