Untreated (–) and treated (+) unboiled MDA-MB-231 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

HRH1 antibody [HL3037] detects HRH1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: HRH1 stained by HRH1 antibody [HL3037] (GTX640475) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Boiled and unboiled MDA-MB-231 whole cell (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.