APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against human HRH1 Intracellular domain.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment encompassing a sequence within the Intracellular domain of human HRH1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
histamine receptor H1 , H1-R , H1R , HH1R , hisH1
Cellular Localization
Cell membrane
Background
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. It has been associated with multiple processes, including memory and learning, circadian rhythm, and thermoregulation. It is also known to contribute to the pathophysiology of allergic diseases such as atopic dermatitis, asthma, anaphylaxis and allergic rhinitis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2015]
Database
Research Area
DATA IMAGES
![Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475](/templates/default/js/lightbox/images/loading.gif "Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475")
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GTX640475 WB Image
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX640475 WB Image
Boiled and unboiled MDA-MB-231 whole cell (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX640475 ICC/IF Image
HRH1 antibody [HL3037] detects HRH1 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: HRH1 stained by HRH1 antibody [HL3037] (GTX640475) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX640475 WB Image
Untreated (–) and treated (+) unboiled MDA-MB-231 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
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REFERENCE
There are currently no references for HRH1 antibody [HL3037] (GTX640475). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HRH1 antibody [HL3037] (GTX640475). Be the first to share your experience with this product.
![Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475](/upload/website/prouct_img/small/GTX640475/GTX640475_T-45432_20240705_WB_B_ub_24070822_953.webp "Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475")
![Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475](/upload/website/prouct_img/small/GTX640475/GTX640475_T-45432_20240705_WB_ub_24070822_136.webp "Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475")
![Anti-HRH1 antibody [HL3037] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640475](/upload/website/prouct_img/small/GTX640475/GTX640475_T-45432_20240628_ICC_IF_B_24070822_362.webp "Anti-HRH1 antibody [HL3037] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640475")
![Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475](/upload/website/prouct_img/small/GTX640475/GTX640475_T-45432_20240705_WB_treatment_PNGaseF_24070822_842.webp "Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475")
![Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475](/upload/website/prouct_img/normal/GTX640475/GTX640475_T-45432_20240705_WB_B_ub_24070822_953.webp "Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475")
![Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475](/upload/website/prouct_img/normal/GTX640475/GTX640475_T-45432_20240705_WB_ub_24070822_136.webp "Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475")
![Anti-HRH1 antibody [HL3037] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640475](/upload/website/prouct_img/normal/GTX640475/GTX640475_T-45432_20240628_ICC_IF_B_24070822_362.webp "Anti-HRH1 antibody [HL3037] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640475")
![Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475](/upload/website/prouct_img/normal/GTX640475/GTX640475_T-45432_20240705_WB_treatment_PNGaseF_24070822_842.webp "Anti-HRH1 antibody [HL3037] used in Western Blot (WB). GTX640475")