Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody (GTX101144) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HSC70 antibody detects HSC70 protein at nucleolus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
HSC70 antibody (GTX101144) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using HSC70(GTX101144) antibody at 1:200 dilution.

HSC70 antibody detects HSC70 protein at nucleolus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
HSC70 antibody (GTX101144) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

HSC70 antibody detects HSC70 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
HSC70 stained by HSC70 antibody (GTX101144) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX101144 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody (GTX101144) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HSC70 antibody (GTX101144) at 1:300 dilution.