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HSD3a antibody [N1C2]

Anti-HSD3a antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103613
Anti-HSD3a antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103613
Anti-HSD3a antibody [N1C2] used in Western Blot (WB). GTX103613

Cat. No. GTX103613

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HSD3a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSD3a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949622

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldo-keto reductase family 1 member C4 , 3-alpha-HSD , C11 , CDR , CHDR , DD-4 , DD4 , HAKRA

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HSD3a antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103613

GTX103613 ICC/IF Image

HSD3a antibody [N1C2] detects HSD3a protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSD3a protein stained by HSD3a antibody [N1C2] (GTX103613) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-HSD3a antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103613

GTX103613 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AKR1C4(GTX103613) antibody at 1:100 dilution.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSD3a antibody [N1C2] used in Western Blot (WB). GTX103613

GTX103613 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
HSD3a antibody
GTX103613 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169