Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody [GT6310] (GTX634459) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

HSP27 antibody [GT6310] detects HSP27 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
HSP27 stained by HSP27 antibody [GT6310] (GTX634459) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

HSP27 antibody [GT6310] detects HSP27 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSP27 stained by HSP27 antibody [GT6310] (GTX634459) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

HSP27 antibody [GT6310] detects HSP27 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
HSP27 stained by HSP27 antibody [GT6310] (GTX634459) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody [GT6310] (GTX634459) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.