IHC-P analysis of human tonsil tissue using GTX25478 HSP60 antibody [4B9/89].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

IP analysis of HeLa cell lysates using GTX25478 HSP60 antibody [4B9/89].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

ICC/IF analysis of A2058 Cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

ICC/IF analysis of ATDC5 Cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

WB analysis of 50μg of the indicated whole cell lysates using GTX25478 HSP60 antibody [4B9/89].
Dilution : 1:1000

IHC-P analysis of human kidney tissue using GTX25478 HSP60 antibody [4B9/89].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

IHC-P analysis of human breast carcinoma tissue using GTX25478 HSP60 antibody [4B9/89].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

ICC/IF analysis of human endothelial cells using GTX25478 HSP60 antibody [4B9/89].

FACS analysis of permeabilized, fixed HeLa cells using GTX25478 HSP60 antibody [4B9/89].
Orange histogram : Primary antibody
Black histogram : Isotype control antibody
Dilution : 10 μg/ml incubated for 1 hour on ice

ICC/IF analysis of HeLa cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

ICC/IF analysis of HeLa and NIH3T3 cells using GTX25478 HSP60 antibody [4B9/89].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

ICC/IF analysis of HeLa cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature