Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed A549, using HSP70 1L(GTX111045) antibody at 1:500 dilution.

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX111045 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using HSP70 1L(GTX111045) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

HSP70 1L antibody [N1N3] detects HSP70 1L protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSP70 1L protein stained by HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:400.
Blue: Hoechst 33342 staining.