Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HTRA2 antibody (GTX110703) diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HTRA2(GTX110703) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

HTRA2 antibody detects HTRA2 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
HTRA2 antibody (GTX110703) dilution: 1:1000