Haploid sperm cell-specific antigen antibody [TRA54]

Cat. No. GTX00716
References 3
Cat. No.  GTX00716
Orthogonal
Anti-Haploid sperm cell-specific antigen antibody [TRA54] used in IHC (Paraffin sections) (IHC-P). GTX00716
GTX00716 IHC-P Image

IHC-P analysis of mouse testis tissue section (C57BL/6) using GTX00716 Haploid sperm cell-specific antigen antibody [TRA54].
Fixation : Bouin's solution

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Anti-Haploid sperm cell-specific antigen antibody [TRA54] used in IHC (Paraffin sections) (IHC-P). GTX00716
GTX00716 IHC-P Image

IHC-P analysis of mouse testis tissue section (8-month) using GTX00716 Haploid sperm cell-specific antigen antibody [TRA54]. Anti-GATA antibody stains nuclei of Sertoli cells in seminiferous tubule while anti-TRA54 antibody stains reproductive cells.
Dilution : 1:50
Antigen retrieval : 10 mM citrate buffer (pH 6.0) for 15min
Fixation : 4% PFA

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Anti-Haploid sperm cell-specific antigen antibody [TRA54] used in Western Blot (WB). GTX00716
GTX00716 WB Image
Orthogonal

WB analysis of various mouse tissue samples using GTX00716 Haploid sperm cell-specific antigen antibody [TRA54].
Dilution : 1 μg/ml
Loading : 100 μg

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Anti-Haploid sperm cell-specific antigen antibody [TRA54] used in IHC (Paraffin sections) (IHC-P). GTX00716
Anti-Haploid sperm cell-specific antigen antibody [TRA54] used in IHC (Paraffin sections) (IHC-P). GTX00716
Anti-Haploid sperm cell-specific antigen antibody [TRA54] used in Western Blot (WB). GTX00716
  • Host
    Rat
  • Clonality
    Monoclonal
  • Clone Name
    TRA54
  • Isotype
    IgM
  • Applications
    WB ICC/IF IHC-P IHC-Fr IP Dot EM
  • Reactivity
    Mouse, Rat
Summary
Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organelles in germ cell cytoplasm from spermatocytes to spermatids. Western blots using TRA54 revealed broad bands with 85 kD, 190 kD and >200 kDa in testis probably due to alternative splicing and/or post-translational modification. The molecular size diversity is due to the difference in glycosylation.
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