Wild-type (WT) and HMOX1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Heme Oxygenase 1 antibody [HL1780] detects secreted Heme Oxygenase 1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded cat spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Heme Oxygenase 1 antibody [HL1780] detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Heme Oxygenase 1 antibody [HL1780] detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (GTX637432) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.