APPLICATION
Application Note
For FACS: Use at a concentration of 10 μg/ml. Foe WB: Use at a concentration of 4 μg/ml. Detects a band of approximately 32 kDa. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Positive Control
Recombinant Human or Rat HO-1 (Hsp32) Protein
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide: MERPQPDSMPQDLSEALKEATKEVHTQAEN, corresponding to amino acids 1-30 of Human Heme Oxygenase 1.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10
Cellular Localization
Microsome , Endoplasmic reticulum membrane
Background
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX13248 IHC-P Image
Immunohistochemistry analysis of human spleen tissue stained with HO-1, mAb (HO-1-1) at 10μg/ml.
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GTX13248 WB Image
Western blot analysis of HO-1 (Hsp32), mAb (HO-1-1)
Lane 1: MW marker
Lane 2: HO-1 (rat), (recombinant)
Lane 3: HO-1 (human), (recombinant)
Lane 4: HO-2 (human), (recombinant)
Lane 5: MDBK cell lysate
Lane 6: Dog liver microsome
Lane 7: Mouse liver microsome.
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REFERENCE
There are currently no references for Heme Oxygenase 1 antibody [HO-1-1] (GTX13248). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Heme Oxygenase 1 antibody [HO-1-1] (GTX13248). Be the first to share your experience with this product.