Hemoglobin alpha antibody [GT1234]

Cat. No. GTX02831

Host	Rabbit
Clonality	Monoclonal
Clone Name	GT1234
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($419)

See all Hemoglobin alpha products

Datasheet

View product citations for antibody GTX02831 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
IHC-P	1:50 - 1:200

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Hemoglobin subunit alpha

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hemoglobin subunit alpha 1 , ECYT7 , HBA-T3 , HBH , METHBA

Background

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3039 HBA1
UniProt: P69905 HBA1

Research Area

DATA IMAGES

GTX02831 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin alpha [GT1234] (GTX02831) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX02831 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin alpha [GT1234] (GTX02831) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX02831 IHC-P Image

IHC-P analysis of mouse spleen tissue section using Hemoglobin alpha [GT1234] Hemoglobin alpha [GT1234].
Dilution : 1:100
Blue : DAPI for nuclear staining.

GTX02831 IHC-P Image

IHC-P analysis of mouse lung tissue section using GTX02831 Hemoglobin alpha [GT1234].
Dilution : 1:100

GTX02831 IHC-P Image

IHC-P analysis of human spleen tissue section using Hemoglobin alpha [GT1234] Hemoglobin alpha [GT1234].
Dilution : 1:100
Blue : DAPI for nuclear staining.

GTX02831 IHC-P Image

IHC-P analysis of rat kidney tissue section using GTX02831 Hemoglobin alpha [GT1234].
Dilution : 1:100

GTX02831 IHC-P Image

IHC-P analysis of human placenta tissue section using GTX02831 Hemoglobin alpha [GT1234].
Dilution : 1:100

GTX02831 WB Image

WB analysis of rat liver tissue lysates using GTX02831 Hemoglobin alpha [GT1234].
Dilution : 1:1000
Loading : 25μg

GTX02831 IHC-P Image

IHC-P analysis of rat spleen tissue section using Hemoglobin alpha [GT1234] Hemoglobin alpha [GT1234].
Dilution : 1:100
Blue : DAPI for nuclear staining.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 419

Qty

Hemoglobin alpha antibody [GT1234]

Cat. No. GTX02831

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Background

Database

Research Area

DATA IMAGES

GTX02831 WB Image

GTX02831 WB Image

GTX02831 IHC-P Image

GTX02831 IHC-P Image

GTX02831 IHC-P Image

GTX02831 IHC-P Image

GTX02831 IHC-P Image

GTX02831 WB Image

GTX02831 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.