Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin beta antibody (GTX116412) diluted at 1:500.

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin beta antibody (GTX116412) diluted at 1:500.

Untreated (–) and treated (+) mouse tissue extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin beta antibody (GTX116412) diluted at 1:500. (CFA: Complete Freunds adjuvant)