Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [HL2457] (GTX638775) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Hemoglobin epsilon antibody [HL2457] detects Hemoglobin epsilon protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2457] (GTX638775) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Hemoglobin epsilon antibody [HL2457] detects secreted Hemoglobin epsilon protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2457] (GTX638775) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Hemoglobin epsilon antibody [HL2457] detects secreted Hemoglobin epsilon protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2457] (GTX638775) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min