Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (GTX108396) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (GTX108396) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (GTX108396) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Untreated (–) and treated (+) mouse tissue extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (GTX108396) diluted at 1:500. (CFA: Complete Freunds adjuvant)