Detection of HCV core protein by immunofluorescence antibody staining. Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA. After incubation for 48 hr, the cells were fixed with acetone and HCV core protein was detected by indirect immunofluorescence staining using this antibody.

Western blotting of HCV core protein. Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA and were subjected to Western blotting using this antibody. The core protein is detected as a 22-kDa band.

Immunohistochemical detection of HCV core protein. Tissue section from a patient with chronic hepatitis C was immunostained to reveal cells expressing HCV core antigen, which are scattered in the lobules (indirect immunohistochemical method, counterstained with Mayer’s hematoxylin).