Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Her2 / ErbB2 (phospho Tyr1139) antibody [HL3272] (GTX640926) diluted at 1:50000 and Her2 / ErbB2 antibody (GTX100509) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Her2 / ErbB2 (phospho Tyr1139) antibody [HL3272] detects Her2 / ErbB2 (phospho Tyr1139) protein by immunofluorescent analysis.
Sample: Mock and treated A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Her2 / ErbB2 (phospho Tyr1139) stained by Her2 / ErbB2 (phospho Tyr1139) antibody [HL3272] (GTX640926) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).