Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] (GTX636940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] detects Her2 / ErbB2 (phospho Tyr1248) protein by immunofluorescent analysis.
Sample: Mock and treated MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Her2 / ErbB2 (phospho Tyr1248) stained by Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] (GTX636940) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] detects Her2 / ErbB2 (phospho Tyr1248) protein at cell membrane by immunofluorescent analysis.
Sample: Mock and treated A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Her2 / ErbB2 (phospho Tyr1248) stained by Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] (GTX636940) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] detects Her2 / ErbB2 (phospho Tyr1248) protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Her2 / ErbB2 (phospho Tyr1248) stained by Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] (GTX636940) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] detects Her2 / ErbB2 (phospho Tyr1248) protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Her2 / ErbB2 (phospho Tyr1248) stained by Her2 / ErbB2 (phospho Tyr1248) antibody [HL1466] (GTX636940) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min