Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody (GTX111525) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody (GTX111525) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hk2 antibody (GTX111525) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Hexokinase II antibody detects Hexokinase II protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Hexokinase II antibody (GTX111525) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Hexokinase II antibody detects HK2 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Hexokinase II antibody (GTX111525) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Hexokinase II antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Hexokinase II stained by Hexokinase II antibody (GTX111525) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Hexokinase II antibody detects Hexokinase II protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Hexokinase II antibody (GTX111525) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat stomach by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
Hexokinase II antibody (GTX111525) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the 2022 in Int J Mol Sci. PMID: 35328751