Dot blot analysis of 100 ng BSA-conjugated Histamine, using Histamine antibody (GTX134710) at 1:4000, 1:8000, 1:16000 and 1:32000.

ELISA plate is coated with 100 μL of Histamine-carrier protein conjugate at 10 μg/mL. The coated ligand is detected with Histamine antibody (GTX134710) at concentrations ranging from 440 ng/mL down to 1.6 ng/mL. Rabbit IgG antibody (HRP) (GTX213110-01) is diluted at 1:2,000 and used to detect the primary antibody.

Histamine antibody detects Histamine protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histamine stained by Histamine antibody (GTX134710) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).