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Histamine antibody

Anti-Histamine antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134710
Anti-Histamine antibody used in ELISA (ELISA). GTX134710
Anti-Histamine antibody used in Dot blot (Dot). GTX134710

Cat. No. GTX134710

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, Dot, ELISA

Reactivity

 Rat
Package
100 μl ($399),
25 μl ($169)
See all Histamine products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
Dot Assay dependent
ELISA 1:1000-1:10000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Histamine

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887326

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histamine antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134710

GTX134710 ICC/IF Image

Histamine antibody detects Histamine protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histamine stained by Histamine antibody (GTX134710) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Histamine antibody used in ELISA (ELISA). GTX134710

GTX134710 ELISA Image

ELISA plate is coated with 100 μL of Histamine-carrier protein conjugate at 10 μg/mL. The coated ligand is detected with Histamine antibody (GTX134710) at concentrations ranging from 440 ng/mL down to 1.6 ng/mL. Rabbit IgG antibody (HRP) (GTX213110-01) is diluted at 1:2,000 and used to detect the primary antibody.

Anti-Histamine antibody used in Dot blot (Dot). GTX134710

GTX134710 Dot Image

Dot blot analysis of 100 ng BSA-conjugated Histamine, using Histamine antibody (GTX134710) at 1:4000, 1:8000, 1:16000 and 1:32000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169