Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (GTX115955) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Histone H2B antibody detects Histone H2B protein at nucleus in mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
Histone H2B antibody (GTX115955) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Histone H2B(GTX115955) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H2B antibody detects Histone H2B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: Histone H2B protein stained by Histone H2B antibody (GTX115955) diluted at 1:500.
Blue: Hoechst 33342 staining.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (GTX115955) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The equivalent of 4 larvae was loaded
A: 120hpf larvae
4-12% NuPAGE Bis Tris precast gel
GTX115955 diluted at 1:1000