Histone H3 antibody [RM188]

Cat. No. GTX60888

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM188
Isotype	IgG
Application	WB, ICC/IF, ELISA, Sandwich ELISA
Reactivity	Human
Package	100 μg ($449)

See all Histone H3 products

Datasheet

View product citations for antibody GTX60888 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.01 μg/mL - 0.25 μg/mL
ICC/IF	1 μg/mL - 5 μg/mL
ELISA	0.2 μg/mL - 1 μg/mL
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX60888, Detection : GTX60885/GTX60886 or Capture : GTX60883, Detection : GTX60888. Please notice that GTX60885/GTX60886/GTX60888 need to be conjugated to Biotin to function as the detection antibody when paired with corresponding capture antibodies. Biotin-conjugated form are not provided.

Not tested in other applications.

Product Note

This antibody reacts to all Histone H3 proteins, independent of post-translational modifications. No cross reactivity with other histone proteins

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide corresponding to the C-terminus of human Histone H3.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60888 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60888 Histone H3 antibody [RM188].
Red : Primary antibody
Green : Actin

GTX60888 ELISA Image

Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody and GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.

GTX60888 ELISA Image

Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156] (5 ug/mL) as the capture antibody and biotinylated GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the detection antibody. Please notice that GTX60888 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60883. Biotin-conjugated form is not provided.

GTX60888 Image

The GTX60888 reacts to both Histone H3.1 and Histone H3.3.

GTX60888 ELISA Image

Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166] (2 ug/mL) as the detection antibody and GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody. Please notice that GTX60886 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.

GTX60888 ELISA Image

Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the detection antibody and GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156] (5 ug/mL) as the capture antibody. Please notice that GTX60888 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60883. Biotin-conjugated form is not provided.

GTX60888 WB Image

WB analysis of recombinant histone H3.3 and histone H3.1 proteins, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates using GTX60888 Histone H3 antibody [RM188].
Dilution : 0.025μg/ml

GTX60888 ELISA Image

Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody and biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 449

Qty

Histone H3 antibody [RM188]

Cat. No. GTX60888

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Research Area

DATA IMAGES

GTX60888 ICC/IF Image

GTX60888 ELISA Image

GTX60888 ELISA Image

GTX60888 Image

GTX60888 ELISA Image

GTX60888 ELISA Image

GTX60888 WB Image

GTX60888 ELISA Image

REFERENCE

REVIEW

Product

Research

Support

Company

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