ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130].
Red : Primary antibody
Green : Actin

The GTX60874 reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.

WB analysis of acid extracts from HeLa cellsuntreated (-) or treated with sodium butyrate (+) using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130].
Dilution : 0.5μg/ml

ChIP analysis of HeLa cells using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg

The binding specificity of RM130 to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.