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Histone H3K27me3 (tri-methyl Lys27) antibody

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in ChIP assay (ChIP assay). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Dot blot (Dot). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Western Blot (WB). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

Cat. No. GTX54106

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, Dot, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Dot Assay dependent
ChIP assay 1:20 - 1:100
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2631277

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in ChIP assay (ChIP assay). GTX54106

GTX54106 ChIP assay Image

ChIP analysis of 293T cell lysate using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

GTX54106 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

GTX54106 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106

GTX54106 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106

GTX54106 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Dot blot (Dot). GTX54106

GTX54106 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Western Blot (WB). GTX54106

GTX54106 WB Image

WB analysis of various sample lysates using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106

GTX54106 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

GTX54106 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:100

REFERENCE

Application Reference

Fan CS et al. Oncotarget 2018; 9 (4) : 4998-5015 Osteopontin-integrin engagement induces HIF-1α-TCF12-mediated endothelial-mesenchymal transition to exacerbate colorectal cancer.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399