Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Histone H3 (phospho Ser10) antibody detects HIST1H3A protein by western blot analysis.
A. 30 μg 4T1 whole lysate/extract (untreated)
B. 30 μg 4T1 whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
15% SDS-PAGE
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HIST1H3A antibody histone cluster 1, H3a protein by immunofluorescent analysis. Sample: RFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: histone cluster 1, H3a protein stained by HIST1H3A antibody (GTX128116) diluted at 1:500. Red: Tubulin, alpha 1b protein stained by TUBA1B antibody (GTX11304) diluted at 1:500 . Blue: Hoechst 33342 staining.

Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3S10ph (phospho Ser10) antibody (GTX128116) dilution: 1:100.

Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein at chromosome by confocal immunofluorescent analysis.
Sample: Mitotic HeLa cells were fixed in 2% paraformaldehyde for 30 min.
Green: Histone H3 (phospho Ser10) protein stained by Histone H3 (phospho Ser10) antibody (GTX128116) diluted at 1:500.
Red: alpha Tubulin antibody (GTX11304) diluted at 1:2500.
Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]

Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Cross-linked ChIP was performed with HeLa chromatin extract treated with Nocodazole (100 ng/ml for 24 h) and 5 μg of either control rabbit IgG or anti-Histone H3S10ph (phospho Ser10) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.

Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract (untreated)
B. 30 μg Rat2 whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr)
15% SDS-PAGE
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Histone H3S10ph (phospho Ser10) antibody immunoprecipitates HIST1H3B protein in IP experiments.
IP samples: 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr
A. 30 μg 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of HIST1H3B protein by 4 μg Histone H3S10ph (phospho Ser10) antibody (GTX128116)
15 % SDS-PAGE
The immunoprecipitated HIST1H3B protein was detected by Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H3 (phospho Ser10) antibody detects HIST1H3A protein at nucleus on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3S10ph (phospho Ser10) (GTX128116) antibody dilution: 1:100.

The data was published in the journal Sci Rep in 2016. PMID: 27819330