Histone H3 (phospho Ser28) antibody detects Histone H3 (phospho Ser28) protein at nucleus on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Histone H3 (phospho Ser28) antibody (GTX128953) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Dotblot analysis of anti-Histone H3 (phospho Ser28) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (phospho Ser28) antibody (GTX128953) 1:5000 dilution.
A: Peptide samples of H3S10 un
B: Peptide samples of H3S10ph
C: Peptide samples of H3S28 un
D: Peptide samples of H3S28ph

Histone H3 (phospho S28) antibody detects Histone H3 (phospho S28) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr)
15 % SDS-PAGE
Histone H3 (phospho S28) antibody (GTX128953) dilution: 1:1000

Histone H3 (phospho Ser28) antibody detects Histone H3 (phospho Ser28) protein at nucleus on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Histone H3 (phospho Ser28) antibody (GTX128953) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H3 (phospho Ser28) antibody detects Histone H3 (phospho Ser28) protein at chromosome in assynchronous HeLa cells.
Sample: HeLa cells were fixed in 2% paraformaldehyde for 30 min.
Green: Histone H3 (phospho Ser28) protein stained by Histone H3 (phospho Ser28) antibody (GTX128953) diluted at 1:500.
Blue: Hoechst 33342 staining.