Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody [HL1977] (GTX637870) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Histone H4 antibody [HL1977] detects Histone H4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 stained by Histone H4 antibody [HL1977] (GTX637870) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Histone H4 antibody [HL1977] detects Histone H4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Histone H4 stained by Histone H4 antibody [HL1977] (GTX637870) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H4 antibody [HL1977] detects Histone H4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Histone H4 stained by Histone H4 antibody [HL1977] (GTX637870) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody [HL1977] (GTX637870) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.