Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H4K8ac (acetyl Lys8) antibody [GT478] (GTX633420) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at nucleus in human ovary by immunohistochemical analysis.
Sample: Paraffin-embedded human ovary.
Histone H4K8ac (acetyl Lys8) antibody [GT478] (GTX633420) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4K8ac (acetyl Lys8) antibody (GTX633420) diluted at 1:1000.

Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4K8ac (acetyl Lys8) protein stained by Histone H4K8ac (acetyl Lys8) antibody [GT478] (GTX633420) diluted at 1:400.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102078) diluted at 1:300.
Scale bar = 10 μm.

Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Histone H4K8ac (acetyl Lys8) antibody [GT478] (GTX633420) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min