WB analysis of 50 ug of the indicated whole cell lysates using GTX25439 Hsp70 antibody [3A3].
Dilution : 1:1000

IHC-P analysis of human tonsil tissue using GTX25439 Hsp70 antibody [3A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

IHC-P analysis of human testis tissue using GTX25439 Hsp70 antibody [3A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

ICC/IF analysis of HeLa cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

ICC/IF analysis of MCF-7 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

ICC/IF analysis of NIH-3T3 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

ICC/IF analysis of HeLa and NIH3T3 cells using GTX25439 Hsp70 antibody [3A3].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

IP analysis of HeLa cell lysates using GTX25439 Hsp70 antibody [3A3].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

WB analysis of 20ul of gill tissue lysates from the salt marsh mussel, Guekensia demissa isolated from various coves in Rhode Island (indicated above the lanes) using GTX25439 Hsp70 antibody [3A3].
Dilution : 1:1000