Hsp70 antibody detects Hsp70 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Hsp70 stained by Hsp70 antibody (GTX112963) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Hsp70 antibody detects Hsp70 protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Hsp70 antibody (GTX112963) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp70 antibody (GTX112963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Hsp70 antibody detects Hsp70 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Hsp70 antibody (GTX112963) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Hsp70 antibody detects Hsp70 protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hsp70 stained by Hsp70 antibody (GTX112963) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Hsp70 antibody detects Hsp70 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: Hsp70 stained by Hsp70 antibody (GTX112963) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Hsp70 antibody detects Hsp70 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: Hsp70 stained by Hsp70 antibody (GTX112963) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC