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Hsp90 beta (phospho Ser254) antibody

Orthogonal
Anti-Hsp90 beta (phospho Ser254) antibody used in Western Blot (WB). GTX50240
Anti-Hsp90 beta (phospho Ser254) antibody used in Western Blot (WB). GTX50240

Cat. No. GTX50240

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all Hsp90 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 254 (V-G-S(p)-D-E) derived from human HSP90 beta.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein 90 alpha family class B member 1 , D6S182 , HSP84 , HSP90B , HSPC2 , HSPCB

Cellular Localization

Cytoplasm,Nucleus,Secreted,Cell membrane

Background

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012]

Database

Research Area

DATA IMAGES

Anti-Hsp90 beta (phospho Ser254) antibody used in Western Blot (WB). GTX50240

GTX50240 WB Image

Orthogonal

WB analysis of extracts from HeLa cells treated with calf intestinal phosphatase (CIP) using GTX50240 HSP90 beta (phospho Ser254) antibody.

Anti-Hsp90 beta (phospho Ser254) antibody used in Western Blot (WB). GTX50240

GTX50240 WB Image

WB analysis of extracts from HeLa cells untreated (lane 1) or treated with heatshock (lane 2) using GTX50240 HSP90 beta (phospho Ser254) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399