Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HuC antibody (GTX134128) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HuC antibody detects HuC protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: HuC stained by HuC antibody (GTX134128) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

HuC antibody detects HuC protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HuC stained by HuC antibody (GTX134128) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

HuC antibody detects HuC protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HuC stained by HuC antibody (GTX134128) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

HuC antibody detects HuC protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HuC stained by HuC antibody (GTX134128) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

HuC antibody detects HuC protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HuC stained by HuC antibody (GTX134128) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

HuC antibody detects HuC protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: HuC stained by HuC antibody (GTX134128) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).