Serial dilutions of Human G-CSF protein (active) were added to murine NFS-60 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.

Sandwich ELISA detection of recombinant Human G-CSF protein (active) (GTX138166-pro) using antibodies as below.

Capture: G-CSF antibody [GT21] (GTX641067) (5 μg/mL)
Detection: HRP-conjugated G-CSF antibody [GT22] (GTX641068) (1 μg/mL)
Please notice that GTX641068 needs to be conjugated to HRP to function as the detection antibody when paired with GTX641067. Please contact us for custom HRP-conjugated antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant Human G-CSF protein (active) (GTX138166-pro) (213.9-3.34 nM). Coated protein was probed with G-CSF antibody [GT21] (GTX641067) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

1 μg of GTX138166-pro Human G-CSF protein (active) was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.