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United States (US)

Human IgG1 Lambda chain isotype control

Cat. No. GTX76676

Reference 1

Host

 Human

Isotype

 IgG1

Application

 WB, Dot, ELISA, Immunoassay, Immunodiffusion, Immunoelectrophoresis
Package
1 mg ($369)
See all Human IgG1 Lambda chain isotype control products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Dot Assay dependent
ELISA Assay dependent
Immunoassay Assay dependent
Immunodiffusion Assay dependent
Immunoelectrophoresis Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM TBS pH8.0

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Fractionation/ion-exchange/affinity chromatography
From human plasma

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

The purified immunoglobulin represents a single subclass and light chain type. Purity of immunoglobulin is greater than 95% as determined by reduced SDS-PAGE. Identity of IgG1, lambda is verified by subclass monoclonal antibodies and type specific polyclonal antibodies in immunoelectrophoresis and indirect ELISA assays.

REFERENCE

Application Reference

Grimm S et al. Mol Biotechnol 2011; 48 (3) : 263-76 Ribosome display selection of a murine IgG1 Fab binding affibody molecule allowing species selective recovery of monoclonal antibodies.
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Package List Price ($)
$ 369