Adipogenesis inhibition of 3T3L-1 cells. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1μM, and 10μg/ml, respectively and continued for 2 days (day 0).The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml of human Jagged1-Fc (GTX65636-pro) and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.

Induction of Hes-1 with the treatment of GTX65636-pro Jagged 1 Fc fusion protein. A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5μg/ml of human Jagged1-Fc fusion protein as in indicated time points and each cell lysate was prepared and subjected to western blot by using human Hes1 antibody or GAPDH antibody.