The chemotactic effect of GTX00099-pro Human SDF1 protein (active).
Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without SDF1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 10 ng/ml SDF1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).

SDS-PAGE analysis of GTX00099-pro Human SDF1 protein (active).

WB analysis of GTX00099-pro Human SDF1 protein (active).