Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) in tau K18 P301L monomers (GTX17673-pro) over time. Thioflavin T ex = 450 nm, em = 485 nm.

SDS-PAGE of ~16 kDa Human Tau (K18) protein, mutant P301L (monomer) (GTX17673-pro).
Lane 1: MW Ladder. Lane 2: Human Tau (K18) protein, mutant P301L (monomer) (GTX17673-pro).