Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IFITM1 antibody (GTX101728) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunofluorescence analysis of methanol-fixed HeLa, using IFITM1(GTX101728) antibody at 1:500 dilution.

IFITM1 antibody detects IFITM1 protein at cytosol and membrane on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
IFITM1 antibody (GTX101728) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min