Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody (GTX100521) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody (GTX100521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IGF1 antibody detects IGF1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
IGF1 antibody (GTX100521) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

IGF1 antibody detects IGF1 protein at Golgi apparatus membrane by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IGF1 protein stained by IGF1 antibody (GTX100521) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody (GTX100521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.