WB (peptide competition) analysis of CHO-T cells over-expressing the human insulin receptor stimulated with 100 nM insulin for 10 min at 37ºC (Lane 2-5) using GTX25680 IGF1R beta (phospho Tyr1135 / Tyr1136) / Insulin Receptor beta (phospho Tyr1150 / Tyr1151) antibody prior incubated with the phosphopeptide immunogen (Lane 2), the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (Lane 3), or a generic phosphotyrosine-containing peptide (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

ICC/IF analysis of MCF7 cells treated with insulin (100nM for 5 min) using GTX25680 IGF1R beta (phospho Tyr1135 / Tyr1136) / Insulin Receptor beta (phospho Tyr1150 / Tyr1151) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes