Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody [HL3537] (GTX641434) diluted at 1:1000 and IGF1R beta antibody [HL1958] (GTX637795) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody [HL3537] detects IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) protein by immunofluorescent analysis.
Sample: Mock and treated 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) stained by IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody [HL3537] (GTX641434) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).