Sandwich ELISA detection of recombinant E.coli expressed, mature recombinant human IGF2 protein using antibodies as below.

Capture: IGF2 antibody [HL2937] (GTX640321) (5 μg/mL)
Detection: HRP-conjugated IGF2 antibody [HL1982] (GTX637875) (1 μg/mL)
Please notice that GTX637875 needs to be conjugated to HRP to function as the detection antibody when paired with GTX640321. Please contact us for custom HRP-conjugated antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (GTX637875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, mature recombinant human IGF2 protein (533.33-8.33 nM). Coated protein was probed with IGF2 antibody [HL1982] (GTX637875) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (GTX637875) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IGF2 antibody [HL1982] detects IGF2 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IGF2 stained by IGF2 antibody [HL1982] (GTX637875) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (GTX637875) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse plasma (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (GTX637875) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.