IGF2 antibody [HL2937] detects secreted IGF2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
IGF2 stained by IGF2 antibody [HL2937] (GTX640321) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2937] (GTX640321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of recombinant E.coli expressed, mature recombinant human IGF2 protein using antibodies as below.

Capture: IGF2 antibody [HL2937] (GTX640321) (5 μg/mL)
Detection: HRP-conjugated IGF2 antibody [HL1982] (GTX637875) (1 μg/mL)
Please notice that GTX637875 needs to be conjugated to HRP to function as the detection antibody when paired with GTX640321. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of recombinant E.coli expressed, mature recombinant human IGF2 protein using antibodies as below.

Capture: IGF2 antibody [HL2937] (GTX640321) (5 μg/mL)
Detection: HRP-conjugated IGF2 antibody [HL2132] (GTX638104) (1 μg/mL)
Please notice that GTX638104 needs to be conjugated to HRP to function as the detection antibody when paired with GTX640321. Please contact us for custom HRP-conjugated antibody.