GeneTex

  • Country / Location Selection

United States (US)

IGF2 antibody [HL2937]

Recombinant antibody Orthogonal
Anti-IGF2 antibody [HL2937] used in ELISA (ELISA). GTX640321
Anti-IGF2 antibody [HL2937] used in ELISA (ELISA). GTX640321
Anti-IGF2 antibody [HL2937] used in Western Blot (WB). GTX640321
Anti-IGF2 antibody [HL2937] used in IHC (Paraffin sections) (IHC-P). GTX640321

Cat. No. GTX640321

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2937

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IGF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX640321, Detection : GTX638104 / GTX637875.
Please notice that the detection antibody needs to be conjugated to HRP to function as the detection antibody when paired with capture antibody.
Please contact us for custom HRP-conjugated antibody.
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human IGF2.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor 2 , C11orf43 , GRDF , IGF-II , PP9974

Cellular Localization

Secreted

Background

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-IGF2 antibody [HL2937] used in ELISA (ELISA). GTX640321

GTX640321 ELISA Image

Sandwich ELISA detection of recombinant E.coli expressed, mature recombinant human IGF2 protein using antibodies as below.

Capture: IGF2 antibody [HL2937] (GTX640321) (5 μg/mL)
Detection: HRP-conjugated IGF2 antibody [HL2132] (GTX638104) (1 μg/mL)
Please notice that GTX638104 needs to be conjugated to HRP to function as the detection antibody when paired with GTX640321. Please contact us for custom HRP-conjugated antibody.

Anti-IGF2 antibody [HL2937] used in ELISA (ELISA). GTX640321

GTX640321 ELISA Image

Sandwich ELISA detection of recombinant E.coli expressed, mature recombinant human IGF2 protein using antibodies as below.

Capture: IGF2 antibody [HL2937] (GTX640321) (5 μg/mL)
Detection: HRP-conjugated IGF2 antibody [HL1982] (GTX637875) (1 μg/mL)
Please notice that GTX637875 needs to be conjugated to HRP to function as the detection antibody when paired with GTX640321. Please contact us for custom HRP-conjugated antibody.

Anti-IGF2 antibody [HL2937] used in Western Blot (WB). GTX640321

GTX640321 WB Image

Orthogonal

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2937] (GTX640321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IGF2 antibody [HL2937] used in IHC (Paraffin sections) (IHC-P). GTX640321

GTX640321 IHC-P Image

IGF2 antibody [HL2937] detects secreted IGF2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
IGF2 stained by IGF2 antibody [HL2937] (GTX640321) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for IGF2 antibody [HL2937] (GTX640321). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for IGF2 antibody [HL2937] (GTX640321). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations